重组
RabMAb

Anti-Rad50抗体[EPR3466(2)] (ab124682)

概述

  • 产品名称
    Anti-Rad50抗体[EPR3466(2)]
    参阅全部 Rad50 一抗
  • 描述
    兔单克隆抗体[EPR3466(2)] to Rad50
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide, corresponding to residues in Human Rad50.

  • 阳性对照
    • K562, MOLT4, Jurkat and MCF7 cell lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/80.
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 154 kDa).

靶标

  • 功能
    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 组织特异性
    Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • 疾病相关
    Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • 序列相似性
    Belongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • 结构域
    The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

图片

  • Unpurified ab124682 staining Rad50 in the U2OS cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 5% BSA for 60 minutes. Samples were incubated with undiluted primary antibody  for 2 hours. An undiluted FITC conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : MOLT4 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 154 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

文献

ab124682 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (MEFs, U2OS, HEK 293 and Fibroblasts)
Specification
MEFs, U2OS, HEK 293 and Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 07 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 03 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Treatment
10 Gy IR
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Apr 25 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Nov 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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