概述

  • 产品名称
  • 描述
    兔多克隆抗体to Rad50
  • 经测试应用
    适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Chimpanzee, Ferret, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Rad 50 encoded in part by exons 13 and 14.

  • 阳性对照
    • HeLa cell nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab3623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 1-4 µg/mg of lysate.

靶标

  • 功能
    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 组织特异性
    Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • 疾病相关
    Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • 序列相似性
    Belongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • 结构域
    The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Rad50 with ab3623 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 160 kDa

    Sample: Nuclear extract (50µg) from Hela cells

    Affinity purified Rabbit anti-Rad 50 (ab3623) used at 1:4,000 dilution

     

    Sample: Nuclear extract (50µg) from Hela cells

    Affinity purified Rabbit anti-Rad 50 (ab3623) used at 1:4,000 dilution

文献

This product has been referenced in:
  • Lim SW  et al. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Sci Rep 6:29921 (2016). Rat . Read more (PubMed: 27436514) »
  • Hao H  et al. NQDI-1, an inhibitor of ASK1 attenuates acute perinatal hypoxic-ischemic cerebral injury by modulating cell death. Mol Med Rep 13:4585-92 (2016). WB . Read more (PubMed: 27081917) »

See all 6 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab3623.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册