概述

  • 产品名称Anti-Rad50抗体
    参阅全部 Rad50 一抗
  • 描述
    兔多克隆抗体to Rad50
  • 经测试应用适用于: WB, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Chimpanzee, Ferret, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Rad 50 encoded in part by exons 13 and 14.

  • 阳性对照
    • HeLa cell nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab3623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 1-4 µg/mg of lysate.

靶标

  • 功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 组织特异性Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • 疾病相关Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • 序列相似性Belongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • 结构域The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

Anti-Rad50 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Rad50 with ab3623 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 160 kDa

    Sample: Nuclear extract (50µg) from Hela cells

    Affinity purified Rabbit anti-Rad 50 (ab3623) used at 1:4,000 dilution

     

    Sample: Nuclear extract (50µg) from Hela cells

    Affinity purified Rabbit anti-Rad 50 (ab3623) used at 1:4,000 dilution

Anti-Rad50 antibody (ab3623)参考文献

This product has been referenced in:
  • Krencik R  et al. Dysregulation of astrocyte extracellular signaling in Costello syndrome. Sci Transl Med 7:286ra66 (2015). ICC . Read more (PubMed: 25947161) »
  • Yao X  et al. Estrogen-provided cardiac protection following burn trauma is mediated through a reduction in mitochondria-derived DAMPs. Am J Physiol Heart Circ Physiol 306:H882-94 (2014). Histology ; Rat . Read more (PubMed: 24464748) »

See all 2 Publications for this product

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