Customer reviews and FAQs

概述

  • 产品名称Anti-Rad50抗体
    参阅全部 Rad50 一抗
  • 描述
    兔多克隆抗体to Rad50
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Rad 50 encoded in partby exons 2 and 3.

  • 阳性对照
    • HeLa cell nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab3622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 157 kDa (predicted molecular weight: 150 kDa).
IP Use a concentration of 1 - 4 µg/ml.

靶标

  • 功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 组织特异性Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • 疾病相关Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • 序列相似性Belongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • 结构域The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

Anti-Rad50 antibody 图像

  • Immunoprecipitation - Rad50 antibody (ab3622)
    Immunoprecipitation - Rad50 antibody (ab3622)

    Lanes:

    1. IP from 1mg extract with 1.25g Rabbit anti-Rad 50 (exon 2-3) (ab3622)

    2. IP from 1mg extract with 1.25µg Rabbit anti-Rad 50 (exon 13-14) (ab3623)

    Sample: Nuclear extract from Hela cells

    for Western blot, affinity purified Rabbit anti-Rad 50 (ab3622) used at 1:4000 dilution

Anti-Rad50 antibody (ab3622)参考文献

This product has been referenced in:
  • Goldstein M  et al. Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair. Proc Natl Acad Sci U S A 110:16874-9 (2013). IP, ChIP . Read more (PubMed: 24082117) »
  • Tommiska J  et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer 118:2911-6 (2006). Read more (PubMed: 16385572) »

See all 2 Publications for this product

发表研究结果有使用 ab3622？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Western blot abreview for Anti-Rad50 antibody

 Inconclusive
Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Zebrafish Cell lysate - whole cell (whole embryo 2dpf and 3dpf)
Specification whole embryo 2dpf and 3dpf
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
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Jude Rosenthal

Verified customer

提交于 Aug 29 2014

As I told you on the phone, I need to find out whether the ab3622 rad50 antibody can crossreact with Xenopus (frog) protein. Could you please the corresponding region in Xrad50 is identical to or with high homology with human rad50 peptide antigen sequ...

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Thank you for your enquiry. I have been in contact with the originator of this antibody and this antibody will probably not work with Xenopus rad50. Exact sequence information is proprietary. I hope this information helps, please do not hesitate t...

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Customer is not able to obtain a signal in WB with ab3622. Lot# 93311. Samples are human (Hela, etc) and the antibody was used at 1:1000 with incubation o/n at 4C. Her lab has used multiple vials of this antibody before under the same conditions with t...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab3622. We have not had any other reports of problems with this antibody and since multiple vials have worked for you in the past, my assumption is that the late...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"