Anti-Rad21抗体(Agarose) (ab19205)

概述

  • 产品名称Anti-Rad21抗体(Agarose)
    参阅全部 Rad21 一抗
  • 描述
    兔多克隆抗体to Rad21 (Agarose)
  • 偶联物Agarose
  • 经测试应用适用于: IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide which represents a portion of human Rad21 encoded by exon 14.

  • 常规说明100ug of antibody is conjugated to 200ul of agarose which is provided as a 50% slurry in a 400ul volume.

性能

应用

Our Abpromise guarantee covers the use of ab19205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
  • 应用说明IP: Use at a concentration of 15 - 25 µl/0.1 - 1mg of protein lysate or extract.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
    • 序列相似性Belongs to the rad21 family.
    • 结构域The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.
    • 翻译后修饰Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
      Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.
    • 细胞定位Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDLS4 antibody
      • Double-strand-break repair protein rad21 homolog antibody
      • hHR21 antibody
      • HR21 antibody
      • HRAD21 antibody
      • KIAA0078 antibody
      • MCD1 antibody
      • Nuclear matrix protein 1 antibody
      • NXP-1 antibody
      • NXP1 antibody
      • Protein involved in DNA double-strand break repair antibody
      • RAD21 antibody
      • RAD21 homolog (S. pombe) antibody
      • RAD21 homolog antibody
      • RAD21_HUMAN antibody
      • Scc1 antibody
      • SCC1 homolog antibody
      see all

    Anti-Rad21 antibody (Agarose) (ab19205)参考文献

    ab19205 has not yet been referenced specifically in any publications.

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