RAD18 overexpression 293T裂解液(全细胞) (ab94312)

概述

  • 产品名称RAD18 overexpression 293T裂解液(全细胞)
  • 描述
    RAD18 overexpression裂解液
  • 常规说明ab94312 is a 293T cell transfected lysate in which Human RAD18 has been transiently over-expressed using a pCMV-RAD18 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the RAD18 family. Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.

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应用

Our Abpromise guarantee covers the use of ab94312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

RAD18 overexpression 293T lysate (whole cell) 图像

  • ab94312 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-RAD18 antibody - ChIP Grade (ab57447) at 1/500 dilution

    Lane 1 : RAD18 overexpression 293T lysate (whole cell) (ab94312)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

RAD18 overexpression 293T lysate (whole cell) (ab94312)参考文献

ab94312 has not yet been referenced specifically in any publications.

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