Anti-RAD18抗体- ChIP Grade (ab57447)

概述

  • 产品名称Anti-RAD18抗体- ChIP Grade
    参阅全部 RAD18 一抗
  • 描述
    小鼠单克隆抗体to RAD18 - ChIP Grade
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ChIP, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment: EKEIDEIHSK YRKKHKSEFQ LLVDQARKGY KKIAGMSQKT VTITKEDEST EKLSSVCMGQ EDNMTSVTNH FSQSKLDSPE ELEPDREEDS SSCIDIQEV, corresponding to amino acids 332-431 of Human RAD18

性能

应用

Our Abpromise guarantee covers the use of ab57447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 25 µg/ml.
IHC-P Use a concentration of 1.5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 23038767
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the RAD18 family.
    Contains 1 RING-type zinc finger.
    Contains 1 SAP domain.
    Contains 1 UBZ-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810024C04Rik antibody
    • DNA repair protein rad18 antibody
    • E3 ubiquitin-protein ligase RAD18 antibody
    • EC 6.3.2.- antibody
    • hHR 18 antibody
    • hHR18 antibody
    • hRAD 18 antibody
    • hRAD18 antibody
    • MGC156682 antibody
    • Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
    • Postreplication repair protein hRAD18p antibody
    • Postreplication repair protein RAD18 antibody
    • RAD 18 antibody
    • RAD18 antibody
    • RAD18 homolog (S. cerevisiae) antibody
    • RAD18 homolog antibody
    • RAD18 S. cerevisiae homolog antibody
    • RAD18 S. cerevisiae homolog of antibody
    • RAD18 transcript variant antibody
    • RAD18_HUMAN antibody
    • Rad18sc antibody
    • Radiation sensitivity protein 18 antibody
    • RING finger protein 73 antibody
    • RNF 73 antibody
    • RNF73 antibody
    • Structural maintenance of chromosomes protein 6 antibody
    • YCR066W antibody
    • YCR66W antibody
    see all

Anti-RAD18 antibody - ChIP Grade 图像

  • RAD18 antibody (ab57447) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.


  • Predicted band size : 56 kDa
  • Immunoperoxidase of ab57447 (1.5 ug/ml) staining human testis (formalin-fixed paraffin-embedded).
  • ab57447 (25 ug/ml) staining Hela cells using immunofluorescence.
  • Anti-RAD18 antibody - ChIP Grade (ab57447) + PC12

    Predicted band size : 56 kDa
  • Overlay histogram showing HeLa cells stained with ab57447 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57447, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RAD18 antibody - ChIP Grade (ab57447)参考文献

This product has been referenced in:
  • Peng M  et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB ; Human . Read more (PubMed: 24966277) »
  • Shoaib M  et al. PUB-NChIP--"in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. Genome Res 23:331-40 (2013). ChIP, ICC/IF ; Human . Read more (PubMed: 23038767) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us. Yes, similar to that customer's Abreview, it looks as though you are knocking down the RAD18 that runs at 75 & 85 kDa in reduced/denatured conditions. You could run a native gel and see if there are any differences noticed....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Treatment control or Rad18 specific siRNA for 72hrs
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Grant Stewart

Verified customer

提交于 Jul 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"