概述

  • 产品名称Anti-Rad17抗体
    参阅全部 Rad17 一抗
  • 描述
    山羊多克隆抗体to Rad17
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Gorilla
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Rad 17 encoded within exon 14.

  • 阳性对照
    • HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab2847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500 - 1/15000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).

靶标

  • 功能Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
  • 组织特异性Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
  • 序列相似性Belongs to the rad17/RAD24 family.
  • 翻译后修饰Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
  • 细胞定位Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCYC antibody
    • Cell cycle checkpoint protein antibody
    • Cell cycle checkpoint protein RAD17 antibody
    • FLJ41520 antibody
    • HRAD 17 antibody
    • hRad17 antibody
    • R24L antibody
    • Rad 17 antibody
    • Rad 24 antibody
    • RAD1 (S. pombe) homolog antibody
    • RAD1 homolog antibody
    • RAD17 antibody
    • RAD17 homolog (S. pombe) antibody
    • RAD17 homolog antibody
    • Rad17 like protein antibody
    • RAD17, S. pombe, homolog of antibody
    • RAD17_HUMAN antibody
    • RAD17Sp antibody
    • Rad24 antibody
    • Rad24, mouse, homolog of antibody
    • Rad24, S. cerevisiae, homolog of antibody
    • RF C activator 1 homolog antibody
    • RF C/activator 1 homolog antibody
    • RF-C/activator 1 homolog antibody
    see all

Anti-Rad17 antibody 图像



  • Predicted band size : 77 kDa


    ab2847 at a 1:10000 dilution staining ~90 kDa Rad 17 in HeLa whole cell lysate (30µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds).

Anti-Rad17 antibody (ab2847)参考文献

This product has been referenced in:
  • Shin MH  et al. ATM-dependent phosphorylation of the checkpoint clamp regulates repair pathways and maintains genomic stability. Cell Cycle 11:1796-803 (2012). Read more (PubMed: 22453082) »

See 1 Publication for this product

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