概述

  • 产品名称Anti-Rad1抗体
    参阅全部 Rad1 一抗
  • 描述
    小鼠单克隆抗体to Rad1
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant tagged fragment: MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL , corresponding to amino acids 1-90 of Human Rad1.

  • 阳性对照
    • HeLa cell lysate. RAD1 transfected 293T cell line. Human lateral ventricle wall tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab76830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

靶标

  • 功能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
  • 组织特异性Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
  • 序列相似性Belongs to the rad1 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell cycle checkpoint protein Hrad1 antibody
    • Cell cycle checkpoint protein Rad 1 A / B antibody
    • Cell cycle checkpoint protein RAD1 antibody
    • Checkpoint control protein HRAD1 antibody
    • Checkpoint control protein RAD1 antibody
    • DNA repair exonuclease antibody
    • DNA repair exonuclease rad1 antibody
    • DNA repair exonuclease rad1 homolog antibody
    • DNA repair exonuclease REC1 antibody
    • DNA repair protein RAD1 antibody
    • EC 3.1.11.2 antibody
    • Exonuclease homolog RAD1 antibody
    • GTP-binding protein RAD antibody
    • hRAD 1 antibody
    • hRAD1 antibody
    • MGC77779 antibody
    • RAD 1 antibody
    • RAD antibody
    • RAD1 antibody
    • RAD1 homolog (S. pombe) antibody
    • RAD1 homolog antibody
    • Rad1 like DNA damage checkpoint antibody
    • Rad1 like DNA damage checkpoint protein antibody
    • RAD1, S. pombe, homolog of antibody
    • Rad1-like DNA damage checkpoint protein antibody
    • RAD1_HUMAN antibody
    • Ras associated with diabetes antibody
    • REC 1 antibody
    • REC1 antibody
    • RRAD antibody
    see all

Anti-Rad1 antibody 图像

  • Anti-Rad1 antibody (ab76830) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Anti-Rad1 antibody (ab76830) at 5 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-Rad1 antibody (ab76830) at 5 µg/ml

    Lane 1 : Rad1 transfected 293T cell line
    Lane 2 : Non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lateral ventricle wall using ab 76830 at 3 µg/ml.

Anti-Rad1 antibody (ab76830)参考文献

ab76830 has not yet been referenced specifically in any publications.

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