概述

  • 产品名称Anti-Rad1抗体
    参阅全部 Rad1 一抗
  • 描述
    兔多克隆抗体to Rad1
  • 经测试应用适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    PLLTQQIQDEDDQYSLVA

    conjugated to KLH via a C-terminal Cysteine residue linker, corresponding to N terminal amino acids 2 - 19 of Human Rad1

  • 阳性对照
    • HeLa cell lysates; Extracts of HL-60 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum protein
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
  • 应用说明IP: 5-10 ul immunoprecipitates RAD1 from HeLa cell lysates.
    WB: 1/250 - 1/500. Detects a band of approximately 30 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
    • 组织特异性Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
    • 序列相似性Belongs to the rad1 family.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cell cycle checkpoint protein Hrad1 antibody
      • Cell cycle checkpoint protein Rad 1 A / B antibody
      • Cell cycle checkpoint protein RAD1 antibody
      • Checkpoint control protein HRAD1 antibody
      • Checkpoint control protein RAD1 antibody
      • DNA repair exonuclease antibody
      • DNA repair exonuclease rad1 antibody
      • DNA repair exonuclease rad1 homolog antibody
      • DNA repair exonuclease REC1 antibody
      • DNA repair protein RAD1 antibody
      • EC 3.1.11.2 antibody
      • Exonuclease homolog RAD1 antibody
      • GTP-binding protein RAD antibody
      • hRAD 1 antibody
      • hRAD1 antibody
      • MGC77779 antibody
      • RAD 1 antibody
      • RAD antibody
      • RAD1 antibody
      • RAD1 homolog (S. pombe) antibody
      • RAD1 homolog antibody
      • Rad1 like DNA damage checkpoint antibody
      • Rad1 like DNA damage checkpoint protein antibody
      • RAD1, S. pombe, homolog of antibody
      • Rad1-like DNA damage checkpoint protein antibody
      • RAD1_HUMAN antibody
      • Ras associated with diabetes antibody
      • REC 1 antibody
      • REC1 antibody
      • RRAD antibody
      see all

    Anti-Rad1 antibody (ab50310)参考文献

    ab50310 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"