概述

  • 产品名称Anti-RACK1抗体
    参阅全部 RACK1 一抗
  • 描述
    兔多克隆抗体to RACK1
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Pig, Xenopus laevis, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    TEQMTLRGT LKGHNG - C

    , conjugated to KLH by a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-16 of Human RACK1

  • 阳性对照
    • Paraformaldehyde-fixed HeLa cells, HEK-293T cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab62735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
IP Use at an assay dependent dilution. Use 5-10 µL.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
  • 序列相似性Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • 结构域The WD repeats domain 5 mediates interaction with TRIM63.
  • 细胞定位Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation-inducing gene 21 protein antibody
    • GBLP_HUMAN antibody
    • Gnb2-rs1 antibody
    • Gnb2l1 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
    • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
    • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
    • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
    • H12.3 antibody
    • HLC-7 antibody
    • Human lung cancer oncogene 7 protein antibody
    • lung cancer oncogene 7 antibody
    • OTTHUMP00000223704 antibody
    • OTTHUMP00000223870 antibody
    • OTTHUMP00000223891 antibody
    • OTTHUMP00000223893 antibody
    • OTTHUMP00000223900 antibody
    • OTTHUMP00000223902 antibody
    • OTTHUMP00000223930 antibody
    • OTTHUMP00000223931 antibody
    • PIG21 antibody
    • Proliferation inducing gene 21 antibody
    • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
    • RACK1 antibody
    • Receptor for activated C kinase 1 antibody
    • Receptor for activated C kinase antibody
    • Receptor of activated protein kinase C 1 antibody
    see all

Anti-RACK1 antibody 图像

  • All lanes : Anti-RACK1 antibody (ab62735) at 1/500 dilution

    Lane 1 : Extract from hek-293T cell line
    Lane 2 : Extract from HeLa cell line
    Lane 3 : Extract from MCF7 cell line
    Lane 4 : Extract from U2OS cell line
    Lane 5 : Extract from Jurkat cell line
    Lane 6 : Extract from RAT1 cell line
    Lane 7 : Extract from PC12 cell line
    Lane 8 : Extract from NIH3T3 cell line

    Secondary
    Goat Anti-Rabbit IgG
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-RACK1 antibody (ab62735)参考文献

This product has been referenced in:
  • Coppari E  et al. A nanotechnological, molecular-modeling, and immunological approach to study the interaction of the anti-tumorigenic peptide p28 with the p53 family of proteins. Int J Nanomedicine 9:1799-813 (2014). WB ; Human . Read more (PubMed: 24748790) »
  • Zheng Y  et al. Interaction of protein inhibitor of activated STAT 2 (PIAS2) with receptor of activated C kinase 1, RACK1. FEBS Lett 586:122-6 (2012). ICC/IF, IP . Read more (PubMed: 22210188) »

See all 3 Publications for this product

Product Wall

Thank you very much for your interest in ab62735.

We would guarantee ab62735 to work in ICC/IF with mouse cells. We have this reference citing previous use for ICC/IF (though not in mouse primary neurons). http://www.ncbi.nlm.nih.gov/pubmed/2...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (BMM)
Loading amount 15 µg
Specification BMM
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"