概述

  • 产品名称Anti-RACGAP1抗体
    参阅全部 RACGAP1 一抗
  • 描述
    兔多克隆抗体to RACGAP1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-172 of Human RACGAP1 (AAH32754).

  • 阳性对照
    • A431 and Raji whole cell lysates; A431 cells; H1299 xenograft

性能

应用

Our Abpromise guarantee covers the use of ab97315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • 组织特异性Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
  • 序列相似性Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Rho-GAP domain.
  • 结构域The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • 翻译后修饰Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CYK4 antibody
    • GAP antibody
    • Gap1 antibody
    • GTPase activating protein antibody
    • HsCYK-4 antibody
    • ID GAP antibody
    • KIAA1478 antibody
    • Male germ cell RacGap antibody
    • MgcRacGAP antibody
    • Protein CYK4 homolg antibody
    • Protein CYK4 homolog antibody
    • Rac GTPase activating protein 1 antibody
    • Rac GTPase-activating protein 1 antibody
    • RACGAP 1 antibody
    • Racgap1 antibody
    • RGAP1_HUMAN antibody
    see all

Anti-RACGAP1 antibody 图像

  • All lanes : Anti-RACGAP1 antibody (ab97315) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 71 kDa
  • ab97315, at 1/200 dilution, staining RACGAP1 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
  • ab97315, at 1/500 dilution, staining RACGAP1 in paraffin-embedded H1299 xenograft by Immunohistochemistry.

Anti-RACGAP1 antibody (ab97315)参考文献

ab97315 has not yet been referenced specifically in any publications.

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