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Our Abpromise guarantee covers the use of ab2270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).Can be blocked with Human RACGAP1 peptide (ab22898).|
|IP||Use at an assay dependent dilution.|
Primary incubation was 1 hour. Detected by chemiluminescence.