概述

  • 产品名称Anti-RAC2抗体
    参阅全部 RAC2 一抗
  • 描述
    山羊多克隆抗体to RAC2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    PQPTRQQKRACSLL

    , corresponding to C terminal amino acids 179-192 of Human RAC2.

  • 阳性对照
    • MOLT4 or liver lysate.
  • 常规说明Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022. Official Gene Symbol - RAC2. GenBank Accession Number – NP_002863. LocusLink ID - 5880.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab2244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.Can be blocked with Human RAC2 peptide (ab22892).
IHC-P Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative.

靶标

  • 功能Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
  • 组织特异性Hematopoietic specific.
  • 疾病相关Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 细胞定位Cytoplasm. Membrane-associated when activated.
  • Information by UniProt
  • 数据库链接
  • 形式Rac2 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • 别名
    • EN 7 antibody
    • EN-7 antibody
    • EN7 antibody
    • GX antibody
    • HSPC 022 antibody
    • HSPC022 antibody
    • p21 Rac 2 antibody
    • p21 Rac2 antibody
    • p21-Rac2 antibody
    • p21Rac2 antibody
    • RAC 2 antibody
    • Rac2 antibody
    • RAC2_HUMAN antibody
    • Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody
    • Ras related C3 botulinum toxin substrate 2 antibody
    • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody
    • Ras related C3 botulinum toxin substrate 3 antibody
    • Ras-related C3 botulinum toxin substrate 2 antibody
    • Rho family small GTP binding protein Rac 2 antibody
    • Rho family small GTP binding protein Rac2 antibody
    • Small G protein antibody
    see all

Anti-RAC2 antibody 图像

  • All lanes : Anti-RAC2 antibody (ab2244) at 0.1 µg/ml

    Lane 1 : Human thymus tissue lysate
    Lane 2 : Human tonsil tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 21 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Immunohistochemical analysis of paraffin embedded Human Tonsil tissue section labelling RAC2 with ab2244 (0.3µg/ml). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.

  • Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + MOLT4 cell lysate at 35 µg

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. 

Anti-RAC2 antibody (ab2244)参考文献

This product has been referenced in:
  • Kawazu M  et al. Transforming mutations of RAC guanosine triphosphatases in human cancers. Proc Natl Acad Sci U S A 110:3029-34 (2013). Human . Read more (PubMed: 23382236) »
  • Tomilov AA  et al. Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice. J Biol Chem 285:1153-65 (2010). WB ; Mouse . Read more (PubMed: 19892704) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

ab2244 has been produced against the synthetic peptide: PQPTRQQKRACSLL, corresponding to C terminal amino acids 179-192 of Human RAC2.

ab93614 has been produced using a proprietary sequence peptide derive...

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Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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Thank you for your enquiry. This antibody has not been tested for cross-reactivity with RAC1 or RAC3. You can BLAST the sequence to check, but I didn't see any hits for RAC1 or RAC3. If you have any additional questions, please contact us again.

Thank you for your question. Our immunogen was PQPTRQQKRACSLL.Cross-reactivity tests have not been done against Rac1 or Rac3, and a BLAST search could only find a significant alignment with RAC2. I hope this information helps you,

This antibody has not been tested for IHC application yet. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"