RabMAb

Anti-RAC1 + Cdc42 (phospho S71)抗体[EP221Y] (ab76535)

概述

  • 产品名称Anti-RAC1 + Cdc42 (phospho S71)抗体[EP221Y]
    参阅全部 RAC1 + Cdc42 一抗
  • 描述
    兔单克隆抗体[EP221Y] to RAC1 + Cdc42 (phospho S71)
  • 特异性This antibody only detects RAC1 + Cdc42 phosphorylated on Serine 71.
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho peptide corresponding to residues surrounding Serine 71 of human RAC1 + Cdc42

  • 阳性对照
    • WB: A431 cells treated with Lambda Phosphatase.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 相关性Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
    • 细胞定位Cell membrane; Lipid anchor; Cytoplasmic side.
    • 数据库链接
    • 别名
      • CDC42Hs antibody
      • Cell division cycle 42 antibody
      • G25K antibody
      • MIG5 antibody
      • Migration inducing gene 5 antibody
      • p21 Rac1 antibody
      • Ras like protein TC25 antibody
      • Small GTP binding protein Cdc42 antibody
      • TC25 antibody
      see all

    Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] 图像

    • All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilution

      Lane 1 : A431 cell lysate treated with Lambda Phosphatase
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)参考文献

    ab76535 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"