概述

  • 产品名称Anti-Rac1抗体
    参阅全部 Rac1 一抗
  • 描述
    兔多克隆抗体to Rac1
  • 特异性ab97732 detects endogenous levels of total Rac1 protein.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non phosphopeptide derived from around amino acids 69-73 of Human Rac1 (

    PLSYP

    ).

  • 阳性对照
    • HeLa cells; A431 cell extracts.

性能

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应用

Our Abpromise guarantee covers the use of ab97732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/0. Predicted molecular weight: 21 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • 组织特异性Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 结构域The effector region mediates interaction with DEF6.
  • 翻译后修饰AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • 细胞定位Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 形式Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
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Anti-Rac1 antibody 图像

  • All lanes : Anti-Rac1 antibody (ab97732) at 1/500 dilution

    Lane 1 : A431 cell extracts with immunizing peptide
    Lane 2 : A431 cell extracts,


    Predicted band size : 21 kDa
  • ab97732 at 1/100 dilution staining Rac1 in methanol-fixed HeLa cells by Immunofluorescence.

Anti-Rac1 antibody (ab97732)参考文献

This product has been referenced in:
  • Montagner A  et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF ; Mouse . Read more (PubMed: 24203162) »

See 1 Publication for this product

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