概述

  • 产品名称Anti-Rac1抗体
    参阅全部 Rac1 一抗
  • 描述
    兔多克隆抗体to Rac1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Rac1.

    (Peptide available as ab101196.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: MCF7; A431.

性能

应用

Our Abpromise guarantee covers the use of ab84838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • 组织特异性Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 结构域The effector region mediates interaction with DEF6.
  • 翻译后修饰AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • 细胞定位Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 形式Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • 别名
    • Cell migration inducing gene 5 protein antibody
    • Cell migration-inducing gene 5 protein antibody
    • MGC111543 antibody
    • MIG5 antibody
    • Migration inducing gene 5 antibody
    • Migration inducing protein 5 antibody
    • p21 Rac1 antibody
    • p21-Rac1 antibody
    • Rac 1 antibody
    • RAC1 antibody
    • RAC1_HUMAN antibody
    • Ras like protein TC25 antibody
    • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody
    • Ras-like protein TC25 antibody
    • Ras-related C3 botulinum toxin substrate 1 antibody
    • Rho family small GTP binding protein Rac1 antibody
    • TC 25 antibody
    • TC25 antibody
    see all

Anti-Rac1 antibody 图像

  • All lanes : Anti-Rac1 antibody (ab84838) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 29 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

Anti-Rac1 antibody (ab84838)参考文献

ab84838 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84838.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"