Anti-Rac1抗体(ab78139)

概述

  • 产品名称Anti-Rac1抗体
    参阅全部 Rac1 一抗
  • 描述
    兔多克隆抗体to Rac1
  • 特异性Recognizes Rac1b specifically. Does not recognize other Rac1 isoforms.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from the internal region of Human Rac1b (the immunogen sequence is not present in the other Rac1 isoforms)

  • 阳性对照
    • WB: HeLa and MCF7 cell lysates. IHC=P: Human colorectal, prostate or squamous cells carcinoma. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab78139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明ICC/IF: 1/50.
    IHC-P: 1/1000. Antigen retrieval using citrate pH6.0 is recommended.
    WB: 1/1000. Detects a band of approximately 22 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
      Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
    • 组织特异性Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
    • 序列相似性Belongs to the small GTPase superfamily. Rho family.
    • 结构域The effector region mediates interaction with DEF6.
    • 翻译后修饰AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    • 细胞定位Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 形式Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
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    Anti-Rac1 antibody 图像

    • All lanes : Anti-Rac1 antibody (ab78139) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate

      Secondary
      Goat anti-Rabbit HRP-conjugated at 1/5000 dilution

      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescence analysis of HeLa cells using ab78139 at 1/50 dilution (yellow) in conjunction with a Donkey anti-Rabbit IgG Cy3 conjugated. Hela cells were fixed with 3.7% paraformaldehyde and permeabilized with a non-ionic detergent. The cells are dual stained with DAPI (blue).
    • Immunohistochemistry analysis of paraffin-embedded Human tubular colorectal carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining.
    • Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining with some stromal background staining.
    • Immunohistochemistry analysis of paraffin-embedded Human squamous cells carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive nuclear staining pattern.

    Anti-Rac1 antibody (ab78139)参考文献

    ab78139 has not yet been referenced specifically in any publications.

    Product Wall

    The correct storage buffer of antibody ab78139 is as follows; 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% Sodium Azide and 30% Glycerol. It does contain Glycerine.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"