Anti-Rac1抗体[0.T.127] (ab33186)

概述

  • 产品名称Anti-Rac1抗体[0.T.127]
    参阅全部 Rac1 一抗
  • 描述
    小鼠单克隆抗体[0.T.127] to Rac1
  • 特异性Recognizes Rac at 21kD.Slight Rac 2 cross-reactivity
  • 经测试应用适用于: IHC-Fr, WB, Flow Cyt, IHC (PFA fixed), ICC/IF, ELISA, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Rac1.

  • 阳性对照
    • WB/IP: rat brain microsomal protein preparation, Mouse brain IHC-P: Mouse intestine tissue

性能

应用

Our Abpromise guarantee covers the use of ab33186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 2 µg/ml. Predicted molecular weight: 21 kDa.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC (PFA fixed) 1/10 - 1/50.
ICC/IF 1/10 - 1/50.
ELISA 1/100 - 1/1000.
IP Use 1µg for 107 cells.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • 组织特异性Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 结构域The effector region mediates interaction with DEF6.
  • 翻译后修饰AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • 细胞定位Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 形式Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • 别名
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    • MGC111543 antibody
    • MIG5 antibody
    • Migration inducing gene 5 antibody
    • Migration inducing protein 5 antibody
    • p21 Rac1 antibody
    • p21-Rac1 antibody
    • Rac 1 antibody
    • RAC1 antibody
    • RAC1_HUMAN antibody
    • Ras like protein TC25 antibody
    • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody
    • Ras-like protein TC25 antibody
    • Ras-related C3 botulinum toxin substrate 1 antibody
    • Rho family small GTP binding protein Rac1 antibody
    • TC 25 antibody
    • TC25 antibody
    see all

Anti-Rac1 antibody [0.T.127] 图像

  • Anti-Rac1 antibody [0.T.127] (ab33186) at 0.3 µg/ml + Rat Brain Microsomes at 40 µg

    Predicted band size : 21 kDa
  • ab33186 staining Rac1 in Mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA 1 hour at 20°C ; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. An HRP-conjugated Goat anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.

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  • Anti-Rac1 antibody [0.T.127] (ab33186) at 1/750 dilution (for 14 hours at 4°C) + Mouse brain tissue lysate at 15 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 21 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C

    See Abreview

Anti-Rac1 antibody [0.T.127] (ab33186)参考文献

This product has been referenced in:
  • Yoshida S  et al. Growth factor signaling to mTORC1 by amino acid-laden macropinosomes. J Cell Biol 211:159-72 (2015). Read more (PubMed: 26438830) »
  • Skvortsov S  et al. Rac1 as a potential therapeutic target for chemo-radioresistant head and neck squamous cell carcinomas (HNSCC). Br J Cancer 110:2677-87 (2014). Human . Read more (PubMed: 24786604) »

See all 17 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 01 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (16%)
Sample Mouse Cell lysate - whole cell (Primary bone marrow derived macrophage)
Specification Primary bone marrow derived macrophage
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 16 2014



With regards to the specificity of this antibody, its immunogen is recombinant protein containing the full length human Rac. Unfortunately we have not determined the epitope and therefore do not know to exactly whihc part of the protein it bi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% acrylamide gel)
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Enzymatic
Sample Rat Tissue sections (GONAD)
Specification GONAD
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 23 2013

Thank you for contacting us.


This antibody has not been epitope mapped.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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Thank you for taking time to complete the questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Thank you for contacting us. The other positive control lysates you can use are Hela, MCF7, A431 or Raji cell lysates, or Human tubular colorectal, prostate and squamous cells carcinoma tissue lysates. I hope this information is helpful ...

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