兔specific HRP/DAB Detection IHC试剂盒(ab64261)
概述
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产品名称
兔specific HRP/DAB Detection IHC试剂盒
参阅全部 HRP/DAB detection kit 试剂盒 -
产品概述
Rabbit specific HRP/DAB IHC Detection Kit ab64261 is a complete kit to detect a primary antibody raised in rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB. -
说明
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264
- Anti-Mouse HRP-DAB IHC Kit ab64259For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469
Alternatively, see HRP-AEC IHC detection kits.Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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经测试应用
适用于: IHC-P, IHC-Frmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 15 ml 50x DAB Chromogen 1 x 0.5ml Biotinylated goat anti-rabbit IgG(H+L) 1 x 15ml DAB substrate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
研究领域
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相关性
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
图片
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Immunohistochemical analysis staining TNF or IL6 in mouse tissue sections. Samples are incubated in murine TNF (ab9739) or IL6 primary antibody. Bound primary antibodies were detected using IHC detection kit (ab64261). Immunohistochemistry was used to determine the effect of uridine on synovial expression of TNF and IL6.
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Immunohistochemical analysis of KLF4 and pVHL expression in colon cancer tissues. Sections from colon cancer and adjacent tissues were analyzed by immunostaining against KLF4 and pVHL. Staining without primary antibody served as negative control.
Tissue sections were de-waxed with xylene and rehydrated through gradient ethanol into water. For antigen retrieval, sections were heated in citrate buffer (pH6.0) for 10 min at 95°C in a microwave oven. After cooling to room temperature, the sections were then digested with 0.05% trypsin for 10 min at 37°C. Endogenous peroxidase activity was quenched with 0.3% H2O2 in methanol for 30 min at room temperature. After PBS washes, nonspecific antibody binding was blocked by pre-incubating slides with 10% normal goat non-immune serum at 37° for 30 min. After blotting off the blocking serum, sections were incubated with primary antibody against LKF4 as well as primary antibody against VHL at 4° overnight. After PBS washes again, sections were incubated with biotinylated secondary antibody at 1:200 dilution for 30 min at room temperature. After incubating with Vectastain ABC reagent (Vector Laboratories, Inc., Burlingame, CA) for 30 min at room temperature, the sections were developed with Anti Rabbit HRP/DAB Detection Kit (ab64261). Sections were washed in running tap water and lightly counterstained with hematoxylin, followed by dehydration and coverslip mounting. Negative controls were obtained by omitting the primary antibody.
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Human colon carcinoma fixed in 10% NBF for 24 hrs and stained with anti p53 antibody using Anti Rabbit HRP/DAB Detection Kit (ab64261).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (162)
ab64261 被引用在 162 文献中.
- Hu D et al. Inhibition of colorectal cancer tumorigenesis by ursolic acid and doxorubicin is mediated by targeting the Akt signaling pathway and activating the Hippo signaling pathway. Mol Med Rep 27:N/A (2023). PubMed: 36382656
- Wang P & Zhang J Persistent expression of NLRP3 in spinal microglia promotes development of lumbar disc degeneration. Front Immunol 13:1064303 (2022). PubMed: 36505450
- Storms N et al. Presence of Myeloperoxidase in Lamellar Tissue of Horses Induced by an Euglycemic Hyperinsulinemic Clamp. Front Vet Sci 9:846835 (2022). IHC-P ; Horse . PubMed: 35359667
- Zanjanchi P et al. Conjugation of VEGFR1/R2-targeting peptide with gold nanoparticles to enhance antiangiogenic and antitumoral activity. J Nanobiotechnology 20:7 (2022). PubMed: 34983556
- Zhu Y et al. The Ability and Mechanism of nHAC/CGF in Promoting Osteogenesis and Repairing Mandibular Defects. Nanomaterials (Basel) 12:N/A (2022). PubMed: 35055231