兔Anti-Sheep IgG H&L (FITC) (ab6743)

概述

  • 产品名称
    兔Anti-Sheep IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 靶标种属
    Sheep
  • 经测试应用
    适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • 免疫原

    Sheep whole molecule

  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 克隆
    多克隆
  • 常规说明

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

文献

This product has been referenced in:
  • Tang J  et al. Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomed Rep 6:627-632 (2017). Read more (PubMed: 28584633) »
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). Read more (PubMed: 24595072) »

See all 8 Publications for this product

客户评价及客户问答

For IHC-P

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
I tried 1:100 and 1:500 dilutions for this antibody. 1:500 works well with an hour incubation at room temperature.
Username

Abcam user community

Verified customer

提交于 Aug 20 2014

Thank you for your inquiry.

Unfortunately, we do not determine the exact molecular weight for our antibodies.

http://www.abcam.com/ab6743: asshown on the datasheet,the fluorochrome/Protein Ratio is2.3 moles DTAF per mole of Rabbit IgG...

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Gracias por tu respuesta.

No tenemos un protocolo concreto para el uso de este anticuerpo mediante immunohistoquímica, sino protocolos generales que pueden servir de guía para los distintos ensayos.

Te adjunto el link ...

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Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en est...

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We have no experience using this secondary antibody in this technique, so I am afraid that I cannot offer any specific advice. However, this Rabbit polyclonal to sheep IgG will certainly work in conjunction with a sheep primary.

Antibodies are usually stable in buffers containing triton-X. I've personally used antibodies in such buffers with no problems. Also, several automated IHC staining systems routinely use such surfactants in their buffer solutions. We have never actuall...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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