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Our Abpromise guarantee covers the use of ab102172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/5000.|
|ICC||1/200 - 1/5000.|
|ELISA||1/10000 - 1/100000. (as primary)|
|WB||1/2000 - 1/20000. (Colorimetric: 1/5000 - 1/30000, Chemiluminescent: 1/10000 - 1/50000).|
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml
Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102172) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 52 kDa
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
ab102172 has not yet been referenced specifically in any publications.