兔Anti-Rat IgG H&L (Biotin) (ab6733)

概述

  • 产品名称兔Anti-Rat IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 描述
    兔多克隆抗体二抗to大鼠IgG - H&L (Biotin)
  • 靶标种属Rat
  • 经测试应用适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • 免疫原

    Rat IgG whole molecule

  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆多克隆
  • 同种��IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
ELISA 1/500000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Rat IgG H&L (Biotin) 图像

  • Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

    See Abreview

  • Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

Rabbit Anti-Rat IgG H&L (Biotin) (ab6733)参考文献

This product has been referenced in:
  • Lucas AR  et al. Myxomavirus anti-inflammatory chemokine binding protein reduces the increased plaque growth induced by chronic Porphyromonas gingivalis oral infection after balloon angioplasty aortic injury in mice. PLoS One 9:e111353 (2014). IHC ; Mouse . Read more (PubMed: 25354050) »
  • Lee HJ  et al. Oral administration of penta-O-galloyl-{beta}-D-glucose suppresses triple-negative breast cancer xenograft growth and metastasis in strong association with JAK1-STAT3 inhibition. Carcinogenesis 32:804-11 (2011). IHC . Read more (PubMed: 21289371) »

See all 6 Publications for this product

Product Wall

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab6733 que vous avez reçu ne fonctionne pas comme attendu.
Comme convenu j'ai mis en place l'envoi d'une unité de ab7103 (http...

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Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab970...

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I recommend ab7096 -- Goat polyclonal to rat IgG H&L (biotin) Pre-Adsorbed

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"