产品名称 兔Anti-Rat IgG H&L (Alkaline Phosphatase) 参阅全部 IgG 二抗
特异性 By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
IHC-P, ICC, ELISA, WB more details
偶联物 Alkaline Phosphatase 性能
存放说明 Shipped at 4°C. Store at +4°C.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000.
1/100 - 1/1000.
1/1000 - 1/10000. (Primary ELISA).
1/1000 - 1/10000. (Chemiluminescent).
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) 图像
Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab102169)
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control ( ab6160) at 1 µg/ml Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/2000 dilution Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/10000 dilution Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
Abcam recommends blocking with 3% milk to reproduce this western blot data.
Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"