By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
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Immunogen affinity purified
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000.
1/100 - 1/1000.
1/1000 - 1/10000. (Primary ELISA).
1/1000 - 1/10000. (Chemiluminescent).
Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab102169)
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control ( ab6160) at 1 µg/ml Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/2000 dilution Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/10000 dilution Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab102169) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size : 50 kDa Observed band size : 52 kDa
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands. Exposure time : 3 minutes
Abcam recommends blocking with 3% milk to reproduce this western blot data.
has not yet been referenced specifically in any publications.
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