兔抗小鼠IgG H&L (HRP) (ab97046)
Key features and details
- Rabbit Anti-Mouse IgG H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
兔抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
免疫原
Chemical/ Small Molecule corresponding to IgG. Purified mouse IgG
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, PBS, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
相关产品
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Substrate reagent
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
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- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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|
ELISA |
1/10000 - 1/100000. (Primary)
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|
WB | (3) |
1/2000 - 1/20000. (Colorimetric). (Chemiluminescent 1/10000 - 1/50000).
|
说明 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. (Primary) |
WB
1/2000 - 1/20000. (Colorimetric). (Chemiluminescent 1/10000 - 1/50000). |
图片
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IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97046, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/20000 dilution
Developed using the -
Formalin-fixed, paraffin embedded human stomach tissue was stained for p53 using a mouse Anti-p53 antibody.
ab97046 was used as secondary antibody at 1/100 dilution. DAB staining.
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Sample: Human Cell lysate - whole cell (human glioma cells), Loading amount: 20 µg, Specification: human glioma cells
Blocking step: Milk as blocking agent for 1 hour, Concentration: 5 %, Temperature: 25°C
Other product details: 1st Ab Dilution 1/5000 anti-actin Ab, Incubation time: 16 hours, Temperature: 4°C
Secondary antibody: ab97046 used at 1:20,000
Bands: Specific: 45 kDa
Notes: primary Ab (anti-actin) was used as a loading control in 6 human glioma cell lines
This image is courtesy of an anonymous Abreview
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (183)
ab97046 被引用在 183 文献中.
- Luo Y et al. Metabolomic analysis and pharmacological validation of the cerebral protective effect of 3,4‑dihydroxybenzaldehyde on cerebral ischemia‑reperfusion injury. Mol Med Rep 27:N/A (2023). PubMed: 36367170
- Yang S et al. Neutrophil Extracellular Traps Delay Diabetic Wound Healing by Inducing Endothelial-to-Mesenchymal Transition via the Hippo pathway. Int J Biol Sci 19:347-361 (2023). PubMed: 36594092
- Vratarić M et al. Fructose diet ameliorate effects of macrophage migration inhibitory factor deficiency on prefrontal cortex inflammation, neural plasticity, and behavior in male mice. Biofactors 49:90-107 (2023). PubMed: 34767656
- Park SR et al. Efficacy of Low-Level Laser Therapy in a Rabbit Model of Rhinosinusitis. Int J Mol Sci 24:N/A (2023). PubMed: 36614203
- Fukuta T et al. One-Step Pharmaceutical Preparation of PEG-Modified Exosomes Encapsulating Anti-Cancer Drugs by a High-Pressure Homogenization Technique. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 36678605