兔Anti-Mouse IgG H&L (HRP) (ab6728)

概述

  • 产品名称兔Anti-Mouse IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 描述
    兔多克隆抗体二抗to小鼠IgG - H&L (HRP)
  • 靶标种属Mouse
  • 经测试应用适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Mouse IgG whole molecule

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
  • 共轭说明Horseradish Peroxidase (HRP)
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent dilution.
ELISA 1/1000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
WB 1/2000 - 1/10000. PubMed: 17200442

Rabbit Anti-Mouse IgG H&L (HRP) 图像

  • ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  • Ab6728 was used at dilution 1/200 with the primary antibody ab81785 in IHC-P. See the review on ab81785.
  • Ab6728 was used at dilution 1/500 with the primary antibody ab3089 in IHC-Fr (PFA perfusion). See the review on ab3089.
  • Ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)参考文献

This product has been referenced in:
  • White CI  et al. Cardiomyocyte and Vascular Smooth Muscle-Independent 11ß-Hydroxysteroid Dehydrogenase 1 Amplifies Infarct Expansion, Hypertrophy, and the Development of Heart Failure After Myocardial Infarction in Male Mice. Endocrinology 157:346-57 (2016). WB . Read more (PubMed: 26465199) »
  • He S  et al. Upregulation of PREX2 promotes the proliferation and migration of hepatocellular carcinoma cells via PTEN-AKT signaling. Oncol Lett 11:2223-2228 (2016). Read more (PubMed: 26998152) »

See all 43 Publications for this product

Product Wall

Abreviews
Application Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab9484 (1:10000)
Username

Mr. Baptiste Jude

Verified customer

提交于 Oct 10 2016

Abreviews
Application Western blot
I used this antibody, when I perform WB.
This antibody is very sensitive.
Dilution concentration that I used is 1/2000 in TBST(5% BSA) for 1hour.
Username

Abcam user community

Verified customer

提交于 May 30 2014

Abreviews
Application Western blot
When I perform WB, that is very sensitive. making band is clearly
Username

Abcam user community

Verified customer

提交于 Jan 23 2014

Abreviews
Application Western blot
strong detection
Username

Abcam user community

Verified customer

提交于 Jul 11 2013

Thank you for your email. I am sorry to hear that customer have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further suggestions for troubleshooting;

- Please try 5...

Read More

Thank you for contacting us. I am sorry to hear these antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I apprec...

Read More

Thank you for your message.

I am pleased to help answer your question.

I can confirm that '1 mg at 2mg/ml' means that the vial contains 1 mg of antibody at a concentration of 2 mg.ml. The concentration is 2 mg/ml. (Therefore the v...

Read More

Thank you for your reply.

I can confirm that for the HRP conjugated antibodies the approach that Irecommended is the correct one. Spining the vial down will prevent free HRP to bind to un-specifically to samples and therefore avoid increased ...

Read More

Thank you for your enquiry. I am sorry to hear the customer has concerns regarding the quality of ab6721, ab6741, ab6789 and ab6728.

As the antibody does contain proteins and other solutes it would not be abnormal to see a little cloudiness ...

Read More

Thank you for contacting Abcam.

I feel that both products are suitable for IHC-P. Both have many specific references and in-depth scientific support data right on the datasheet. They differ mainly in the species in which they were raised and i...

Read More

1-10 of 35 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"