兔Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786)

概述

  • 产品名称
    兔Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 经测试应用
    适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • 最小交叉反应

    Human, Rat more details
  • 偶联物
    DyLight® 550. Ex: 562nm, Em: 576nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    DyLight® 550 replaces DyLight® 549.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.
WB Use at an assay dependent concentration. See below Abreview

图片

  • All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution

    Lane 1 : Rat Hippocampus
    Lane 2 : Rat Hippocampus
    Lane 3 : Rat Hippocampus

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786) at 1/5000 dilution
    Lane 2 : Rabbit Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab98786) at 1/1000 dilution
    Lane 3 : Other Commercial Dye at 1/1000 dilution


    Performed under reducing conditions.

    This image is courtesy of an anonymous abreview.

    See Abreview

  • Anti-HuC/D staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) was used as a secondary antibody at a concentration of 1/500.
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

文献

ab98786 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Red channel is anti-mouse Dy550, label pre-synaptic marker Bassoon. work at dilution of 1:500, incubate in 2%BSA/PBS O/N in Fridge.
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提交于 Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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