兔Anti-Mouse IgG H&L (Biotin) preadsorbed (ab98784)

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概述

  • 产品名称兔Anti-Mouse IgG H&L (Biotin) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    兔多克隆抗体二抗to小鼠IgG - H&L (Biotin)， pre-adsorbed
  • 靶标种属Mouse
  • 经测试应用适用于: IHC-P, ICC/IF, ELISA, WBmore details
  • 最小交叉反应
    Human, Rat more details
  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab98784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/5000.
ICC/IF 1/200 - 1/1000.
ELISA 1/20000 - 1/400000. as primary
WB 1/20000 - 1/400000. chemiluminescent detection

Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed (ab98784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin), pre-adsorbed (ab98784)This image is courtesy of an anonymous Abreview
    Immunohistochemical analysis of dog skin tissue, detecting staining of NQO1 with ab98784.

    Tissue was fixed with formaldehyde and blocked with none for ; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (ab28947 Mouse monoclonal [A180] to NQO1 at 1/175 in BSA in TBS) for 45 minutes. ab98784 (1/500) was used as the secondary antibody.

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Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (ab98784)参考文献

ab98784 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab98784？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Thank you very much for your reply.
I did vary goat anti-mouse IgG concentration, 1:25, 1:50, 1:100. In
all cases, even without primary antibody, I do see signals in
controls. Here are the conditions used:
goat anti-mouse IgG: ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

Since the no primary control is showing staining, it's probably an issue with the secondary antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"