兔Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7074)

概述

  • 产品名称
    兔Anti-Mouse IgG H&L (Biotin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 经测试应用
    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 最小交叉反应

    Human more details
  • 免疫原

    Full length native Mouse IgG (purified).

  • 偶联物
    Biotin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆
    多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • 应用说明
    Dot: Use at an assay dependant dilution.
    ELISA: 1/20000 - 1/80000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 文献

    This product has been referenced in:
    • Grondin Y  et al. Genetic Polymorphisms Associated with Hearing Threshold Shift in Subjects during First Encounter with Occupational Impulse Noise. PLoS One 10:e0130827 (2015). Read more (PubMed: 26121033) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for contacting Abcam.

    You could use the AF555 antibody that you mentioned, but you could also use the following antibody form our catalogue:

    http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-ad...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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