兔Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7074)

概述

  • 产品名称
    兔Anti-Mouse IgG H&L (Biotin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Mouse
  • 经测试应用
    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 最小交叉反应

    Human more details
  • 免疫原

    Full length native Mouse IgG (purified).

  • 偶联物
    Biotin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆
    多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • 应用说明
    Dot: Use at an assay dependant dilution.
    ELISA: 1/20000 - 1/80000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (ab7074)参考文献

    This product has been referenced in:
    • Grondin Y  et al. Genetic Polymorphisms Associated with Hearing Threshold Shift in Subjects during First Encounter with Occupational Impulse Noise. PLoS One 10:e0130827 (2015). Read more (PubMed: 26121033) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting Abcam.

    You could use the AF555 antibody that you mentioned, but you could also use the following antibody form our catalogue:

    http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-ad...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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