兔Anti-Mouse IgG H&L (Biotin) (ab97044)

概述

  • 产品名称兔Anti-Mouse IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用适用于: ELISA, ICC/IF, IHC-P, WBmore details
  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • 共轭说明Biotin/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000 - 1/400000. (Primary).
ICC/IF 1/200 - 1/1000.
IHC-P 1/500 - 1/5000.
WB 1/20000 - 1/400000. (Chemiluminescent).

Rabbit Anti-Mouse IgG H&L (Biotin) (ab97044)参考文献

ab97044 has not yet been referenced specifically in any publications.

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While it is theoretically possible to amplify your signal by using a biotinylated secondary antibody with an anti-biotin tertiary antibody, it is not commonly done. Because streptavidin binds to biotin with a very...

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Thank you for your inquiry. ab64257 is indeed suitable for the detection of Mouse and Rabbit IgG. It can be used as a secondary antibody when used with rabbit or mouse primary antibodies. About ab64256 and ab97044, all of the information we have...

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Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab970...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"