产品名称 兔Anti-Mouse IgG H&L (Alkaline Phosphatase) 参阅全部 IgG 二抗
特异性 By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
ICC, ELISA, IHC-P, WB more details
偶联物 Alkaline Phosphatase 性能
存放说明 Shipped at 4°C. Store at +4°C.
存储溶液 Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
共轭说明 Molar enzyme: antibody protein ratio is 1:1
常规说明 Part of the AbExcel range.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/5000 - 1/50000. (Primary).
1/100 - 1/1000.
1/5000 - 1/50000. (Chemiluminescent).
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) 图像
Western blot - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043)
All lanes :
Anti-beta Actin antibody [mAbcam 8226] - Loading Control (
) at 1 µg/ml
Lanes 1-6 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/5000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/20000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/50000 dilution Developed using the ECL technique Performed under reducing conditions. Exposure time : 20 minutes
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)参考文献
This product has been referenced in:
et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015).
Read more (PubMed: 25786024) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"