AbExcel

兔Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)

概述

  • 产品名称兔Anti-Mouse IgG H&L (Alkaline Phosphatase)
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用适用于: ICC, ELISA, IHC-P, WBmore details
  • 偶联物Alkaline Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • 共轭说明Molar enzyme: antibody protein ratio is 1:1
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Part of the AbExcel range.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
ELISA 1/5000 - 1/50000. (Primary).
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/50000. (Chemiluminescent).

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) 图像

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/5000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/20000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)参考文献

This product has been referenced in:
  • Bergeron KF  et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015). IHC ; Mouse . Read more (PubMed: 25786024) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab97043.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"