Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
Immunogen affinity purified
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Molar enzyme: antibody protein ratio is 1:1
Part of the
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/5000 - 1/50000. (Primary).
1/100 - 1/1000.
1/5000 - 1/50000. (Chemiluminescent).
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) 图像
Western blot - Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043)
All lanes :
Anti-beta Actin antibody [mAbcam 8226] - Loading Control (
) at 1 µg/ml
Lanes 1-6 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/5000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/20000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97043) at 1/50000 dilution Developed using the ECL technique Performed under reducing conditions. Exposure time : 20 minutes
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043)参考文献
This product has been referenced in:
et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015).
Read more (PubMed: 25786024) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"