兔抗小鼠IgG H&L (Alkaline Phosphatase) (ab97043)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
概述
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产品名称
兔抗小鼠IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Mouse -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
经测试应用
适用于: ICC, ELISA, IHC-P, WBmore details -
偶联物
Alkaline Phosphatase
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
共轭说明
Molar enzyme: antibody protein ratio is 1:1 -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent dilution.
|
|
ELISA |
1/5000 - 1/50000. (Primary).
|
|
IHC-P |
1/100 - 1/1000.
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|
WB |
1/5000 - 1/50000. (Chemiluminescent).
|
说明 |
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ICC
Use at an assay dependent dilution. |
ELISA
1/5000 - 1/50000. (Primary). |
IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/50000. (Chemiluminescent). |
图片
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All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/5000 dilution
Lanes 3-4 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/20000 dilution
Lanes 5-6 : Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab97043 被引用在 3 文献中.
- Gamon LF et al. Iodide modulates protein damage induced by the inflammation-associated heme enzyme myeloperoxidase. Redox Biol 28:101331 (2020). PubMed: 31568923
- Mao K et al. Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3. Oncol Lett 13:893-897 (2017). PubMed: 28356975
- Bergeron KF et al. Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10. PLoS Genet 11:e1005093 (2015). IHC ; Mouse . PubMed: 25786024