兔IgG,多克隆抗体-同型对照(ab37415)

概述

  • 产品名称
    兔IgG,多克隆抗体-同型对照
    参阅全部 Rabbit 亚类对照
  • 特异性
    This antibody has been selected to be an isotype control as it has no known specificity. Please note that the rabbit IgG Fc region may bind nonspecifically to human tissue. Rabbit IgG is an isotype control used to estimate the non-specific binding of target primary antibodies due to Fc binding or other protein-protein interactions.
  • 经测试应用
    适用于: IHC-P, ICC/IF, ELISA, Flow Cyt, ChIP/Chipmore details

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 纯度
    Affinity purified
  • 纯化说明
    Protein A-purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域
  • 别名
    • rabbit isotype control

应用

Our Abpromise guarantee covers the use of ab37415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 24961933
ICC/IF Use at an assay dependent concentration. PubMed: 23995768
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20673990

图片

  • Histogram showing the fluorescence signal for ADRB2-FITC in PBMCs compared to an Isotype control.

    PBMCs were generated from freshly-drawn blood from healthy human donors. After staining with antibodies against ADRB2 (ab36956) or an isotype control (ab37415), cells were incubated with FITC-labeled secondary anti-rabbit-IgG antibody, and ADRB2 expression was assessed by flow cytometry.

  • ChIP-qPCR for H3K36me2 on the BTF3 locus in NTKO and TKO cells. Methylation enrichment was tested 5 kb upstream of the TSS (5′) and at the TSS (right). ab37415 was used as the isotype contol.

文献

This product has been referenced in:
  • Ruan D  et al. XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer. Stem Cell Reports 10:494-508 (2018). Read more (PubMed: 29337117) »
  • Mahe M  et al. An FGFR3/MYC positive feedback loop provides new opportunities for targeted therapies in bladder cancers. EMBO Mol Med 10:N/A (2018). ChIP . Read more (PubMed: 29463565) »

See all 48 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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