RabMAb

兔IgG,单克隆抗体[EPR25A] -同型对照(Low endotoxin, Azide free) (ab199376)

概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Endotoxin level: <1EU/mg (based on Lumulus Amebocyte lysate assay).
  • 克隆单克隆
  • 克隆编号EPR25A
  • 同种型IgG

    应用

    Our Abpromise guarantee covers the use of ab199376 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.
    IHC-P 1/250.
    Flow Cyt 1/70.
    ICC/IF 1/250.
    IP Use at an assay dependent concentration.

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) 图像

    • Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling KCTD5 with ab194825 at 1/70 dilution (red) compared with ab199376 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab199376 detects no signal in FC.

      Note: ab199376 detects no signal in Flow cytometry.

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeled with ab199376 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing no staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab199376 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Immunohistochemistry analysis of rat colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on rat colon tissue was observed.

    • Immunohistochemistry analysis of mouse colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on mouse colon tissue was observed.

    • Immunohistochemistry analysis of human colon tissue control for testing ab199376 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. An anti-rabbit IgG H&L (HRP) (ab97051) was used as a secondary antibody. Counterstained with hematoxylin. No staining on human colon tissue was observed.

    Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)参考文献

    This product has been referenced in:
    • Kaliberov SA  et al. Retargeted adenoviruses for radiation-guided gene delivery. Cancer Gene Ther N/A:N/A (2016). Flow Cyt . Read more (PubMed: 27492853) »
    • Zhao J  et al. Efficient Differentiation of TBX18(+)/WT1(+) Epicardial-Like Cells from Human Pluripotent Stem Cells Using Small Molecular Compounds. Stem Cells Dev N/A:N/A (2016). Flow Cyt . Read more (PubMed: 27927069) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"