Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
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Immunogen affinity purified
From the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgG.
Specific antibodies were absorpted by incubation with Sepharose-bound human IgM.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized human IgM a minimum of 65% protein bound