兔Anti-Human IgG H&L (FITC) (ab81190)

概述

  • 产品名称兔Anti-Human IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 靶标种属Human
  • 特异性No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • 经测试应用适用于: IHC-Frmore details
  • 免疫原

    Human IgG

  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • 共轭说明The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtation and ion-exchange chromatography to clear unbound conjugate.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明


    Low background fluorescence of normal tissue in contrast to cases of autoimmune desease, depostited immunoglobulin IgG.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/40.

Rabbit Anti-Human IgG H&L (FITC) (ab81190)参考文献

ab81190 has not yet been referenced specifically in any publications.

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