兔Anti-Human IgA alpha chain (FITC) (ab97219)

概述

  • 产品名称
    兔Anti-Human IgA alpha chain (FITC)
    参阅全部 IgA 二抗
  • 靶标种属
    Human
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • 经测试应用
    适用于: ICC/IF, IHC-P, Flow Cytmore details
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • 共轭说明
    F/P ratio is 3-7
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

文献

ab97219 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Flow Cytometry
Background: We wanted to distinguish human B cells into IgM+, IgG+ and IgA+ B cells by staining surface immunoglobulin. Since ab97219 is polyclonal, presence of an antibody targeting the extracellular part of the IgA heavy chain could be suspected but needed to be tested.
Staining: Human peripheral blood-derived PBMC stained with antibodies against CD3, CD19, surface IgG and surface IgA. In the image, lymphocytes are gated on CD3- CD19+
Results: There is a clear population of IgA+ B cells in the expected range. A few possibly IgA+/IgG+ B cells are present too.
Username

Mr. Marc Bigler

Verified customer

提交于 Aug 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册