兔抗山羊IgG H&L (HRP)预吸附二抗(ab97105)
Key features and details
- Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
概述
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产品名称
兔抗山羊IgG H&L (HRP)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Goat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
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经测试应用
适用于: ICC, IHC-P, ELISA, WBmore details -
最小交叉反应
Chicken, Horse, Human, Mouse, Pig, Rat more details -
免疫原
Other Immunogen Type corresponding to Goat IgG.
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偶联物
HRP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97105于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/5000.
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ELISA |
1/10000 - 1/100000. ELISA-Primary
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WB | (1) |
1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000
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说明 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. ELISA-Primary |
WB
1/5000 - 1/50000. WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000 |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab97105 被引用在 10 文献中.
- Reithmair M et al. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance. Methods Mol Biol 2504:113-133 (2022). PubMed: 35467283
- Kirchner B et al. Postprandial transfer of colostral extracellular vesicles and their protein and miRNA cargo in neonatal calves. PLoS One 15:e0229606 (2020). PubMed: 32109241
- Buschmann D et al. Propofol and Sevoflurane Differentially Impact MicroRNAs in Circulating Extracellular Vesicles during Colorectal Cancer Resection: A Pilot Study. Anesthesiology 132:107-120 (2020). PubMed: 31596735
- Mussack V et al. Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research. Biomol Detect Quantif 17:100089 (2019). PubMed: 31194192
- Madan E et al. HIF-transcribed p53 chaperones HIF-1a. Nucleic Acids Res 47:10212-10234 (2019). PubMed: 31538203
- Hermann S et al. Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera. J Extracell Vesicles 8:1670935 (2019). PubMed: 31632620
- Buschmann D et al. Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing. J Extracell Vesicles 7:1481321 (2018). PubMed: 29887978
- Chen T et al. Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p. Cell Prolif N/A:e12548 (2018). PubMed: 30507050
- Cimica V et al. Novel Respiratory Syncytial Virus-Like Particle Vaccine Composed of the Postfusion and Prefusion Conformations of the F Glycoprotein. Clin Vaccine Immunol 23:451-9 (2016). PubMed: 27030590
- Ogawa S et al. Shotgun proteomic analysis of porcine colostrum and mature milk. Anim Sci J 85:440-8 (2014). PubMed: 24450292