兔Anti-Goat IgG H&L (Biotin) preadsorbed (ab7131)

Submit an Abreview Q&A (2)Specific References (1)
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概述

  • 产品名称
    兔Anti-Goat IgG H&L (Biotin) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Goat
  • 经测试应用
    适用于: ICC/IF, Dot blot, ELISA, IHC-P, Immunomicroscopy, WB, Flow Cyt, IHC-Frmore details
  • 最小交叉反应

    Human more details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物
    Biotin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆
    多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution. PubMed: 20838433
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Goat IgG H&L (Biotin) preadsorbed (ab7131)参考文献

This product has been referenced in:
  • Fernandez P  et al. Strain-transcendent immune response to recombinant Var2CSA DBL5-e domain block P. falciparum adhesion to placenta-derived BeWo cells under flow conditions. PLoS One 5:e12558 (2010). Flow Cyt . Read more (PubMed: 20838433) »

See 1 Publication for this product

发表研究结果有使用 ab7131？请让我们知道，以便我们可以引用本数据表中的参考文章。

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2 Abreviews or Q&A

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What information do you require regarding the 3 antibodies? Due to the research we are doing, we don't want to name what the antibodies are raised to, but I can give the following information:

Ab1: mouse monoclonal IgG2A, stored in PBS (pH 7...

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Thank you for your message and for providing this further information.

Reviewing the details, I agree there seems to be some crossreactivity to mouse IgG. I can confirm that this antibody has not specifically been pre absorbedwith mouse, so ...

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We are using ab7131 secondary antibody and it is showing signs of crossreactivity to mouse Ig in ELISA.

Thank you for your telephone call yesterday afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody and have some concerns about crossreactivity to mouse..

I would like to reassure you that thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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