兔Anti-Goat IgG H&L (Biotin) (ab6740)

概述

  • 产品名称
    兔Anti-Goat IgG H&L (Biotin)
    参阅全部 IgG 二抗
  • 靶标种属
    Goat
  • 经测试应用
    适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物
    Biotin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • 克隆
    多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
ICC/IF
WB
  • 应用说明
    Dot: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    IHC-P: 1/100 (PMID 18930986).
    IHC-Fr: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 文献

    This product has been referenced in:
    • Bohr S  et al. Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche. PLoS One 8:e64223 (2013). IHC-P . Read more (PubMed: 23696871) »
    • Campos-Junior PH  et al. The spermatogonial stem cell niche in the collared peccary (Tayassu tajacu). Biol Reprod 86:155, 1-10 (2012). Read more (PubMed: 22262689) »

    See all 7 Publications for this product

    客户评价及客户问答

    Thank you for your enquiry. This antibody has not been preadsorbed against rat tissue. In your case I would recommend ab7124 as this is a donkey polyclonal to goat IgG H&L (biotin-conjugated) pre-adsorbed antibody that has minimum cross reactivity ...

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    Thank you very much for your patience and for the details in which you have provided. I contacted the originator of ab1664 and below is the FACS protocl that they used. I hope this will be of use to you and if you continue to experience difficulty with...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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