兔Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)

概述

  • 产品名称
    兔Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Goat
  • 经测试应用
    适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • 最小交叉反应

    Human more details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物
    Alkaline Phosphatase

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • 共轭说明
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • 克隆
    多克隆
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) preadsorbed (ab7133)参考文献

ab7133 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. 1. How much lysate was loaded? We would suggest 5uL for 10x8cm gel. 2. Try to titer up the secondary antibody. The problem seems like a non-specific binding caused by too much antigen and/or antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册