兔Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6742)

概述

  • 产品名称兔Anti-Goat IgG H&L (Alkaline Phosphatase)
    参阅全部 IgG 二抗
  • 靶标种属Goat
  • 经测试应用适用于: Dot blot, ELISA, ICC/IF, WB, IHC-P, IHC-Frmore details
  • 免疫原

    Goat IgG whole molecule

  • 偶联物Alkaline Phosphatase

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • 共轭说明Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
WB Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) 图像

  • Ab6742 was used at dilution 1/4000 with the primary antibody ab20773 in ELISA. See the review on ab20773.

Rabbit Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6742)参考文献

This product has been referenced in:
  • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). WB . Read more (PubMed: 23223527) »
  • Takayama H  et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA . Read more (PubMed: 18523555) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However...

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Thank you for your enquiry. I see no reason why you can't substitute in a different secondary antibody, but you want to consult the instructions of the kit that you have, or are interested in purchasing. If you have any more questions, please contact u...

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Thank you for sending the details of your protocol and the picture of your blot. I really think that the background bands are due to the secondary. What appears to be happening is that the secondary is binding additional proteins and reacting with the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"