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By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
Our Abpromise guarantee covers the use of ab102345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/200 - 1/5000.|
|IHC-P||1/200 - 1/5000.|
|ELISA||1/10000 - 1/100000. (Primary).|
|WB||1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000).|
ab102345 has not yet been referenced specifically in any publications.