兔F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)

概述

  • 产品名称兔F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • 最小交叉反应
    Human more details
  • 免疫原

    Full length native Mouse IgG (purified).

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 共轭说明R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • 克隆多克隆
  • 常规说明


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.

Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)参考文献

ab7000 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"