兔F(ab')2 Anti-Goat IgG H&L (TRITC) preadsorbed (ab5752)

概述

  • 产品名称兔F(ab')2 Anti-Goat IgG H&L (TRITC) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Goat
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
  • 经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
  • 最小交叉反应
    Human more details
  • 免疫原

    Goat IgG whole molecule.

  • 偶联物TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • 共轭说明Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Rabbit IgG F(ab’)2
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Rabbit F(ab')2 Anti-Goat IgG H&L (TRITC) preadsorbed (ab5752)参考文献

ab5752 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"