兔F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed (ab102348)

概述

  • 产品名称兔F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed
    参阅全部 IgG 二抗
  • 靶标种属Goat
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
  • 经测试应用适用于: IHC-P, ICC, ELISA, WBmore details
  • 最小交叉反应
    Human, Mouse, Rat more details
  • 偶联物Biotin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/5000.
ICC 1/200 - 1/1000.
ELISA 1/10000 - 1/200000. (Primary).
WB 1/10000 - 1/200000. (Chemiluminescent).

Rabbit F(ab')2 Anti-Goat IgG - H&L (Biotin), pre-adsorbed (ab102348)参考文献

ab102348 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Secondary Ab: Biotinylated rabbit anti-Goat F(ab’)2 (Abcam ab102348) Concentration 1:500 30’ RT
Labeling system : Avidin-biotin complex (Vector Lab, Vectastain Elite ABC kit PK-6100) 30’ RT
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提交于 Jul 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"