概述

  • 产品名称兔Anti-Chicken IgY H&L (HRP)
    参阅全部 IgY 二抗
  • 靶标种属Chicken
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用适用于: ICC, IHC-P, ELISA, WBmore details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 共轭说明Molar enzyme/ antibody protein ratio is 4:1
  • 克隆多克隆
  • 同种型IgG
  • 常规说明Part of the AbExcel range.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000

Rabbit Anti-Chicken IgY H&L (HRP) 图像

  • All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Rabbit Anti-Chicken IgY H&L (HRP) (ab97140)参考文献

This product has been referenced in:
  • Nakao LS  et al. Mechanism-based proteomic screening identifies targets of thioredoxin-like proteins. J Biol Chem 290:5685-95 (2015). Read more (PubMed: 25561728) »
  • Thiede-Stan NK  et al. Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex. J Cell Sci 128:3583-96 (2015). Read more (PubMed: 26290381) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab97140.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"