Our Abpromise guarantee covers the use of ab97137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use at an assay dependent dilution. | |
IHC-P | 1/100 - 1/1000. | |
ELISA | 1/5000 - 1/50000. (as Primary). | |
WB | 1/5000 - 1/50000. (Chemiluminescent). |
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.
Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/5000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/20000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/50000 dilution Developed using the ECL technique. Performed under reducing conditions. Exposure time : 12 minutes |
ab97137 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"