概述
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产品名称兔抗鸡IgY H&L (Alkaline Phosphatase)
参阅全部 IgY 二抗 -
宿主Rabbit
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靶标种属Chicken
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特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
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经测试应用适用于: ICC, IHC-P, ELISA, WBmore details
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偶联物Alkaline Phosphatase
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
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共轭说明Molar enzyme: antibody protein ratio is 1:1
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克隆多克隆
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同种型IgG
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常规说明Part of the AbExcel range.
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研究领域
应用
Our Abpromise guarantee covers the use of ab97137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent dilution. | |
| IHC-P | 1/100 - 1/1000. | |
| ELISA | 1/5000 - 1/50000. (as Primary). | |
| WB | 1/5000 - 1/50000. (Chemiluminescent). |
图片
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Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137)All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/5000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/20000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (AP) (ab97137) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 12 minutes
文献
ab97137 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"