This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions.
The activity of each lot is determined using a dot-spot test system [Moeremans et al., J. Immunol., Methods 74, (1984), 353.]
This product is an immunogold reagent built around colloidal gold particles of 10nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coeffiecient of variance for the 6nm particle size conjugate is less than 10%.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also ven Bergen en Henegouwen and Leunissen, Histochemistry, 85,(1986), 81.)
Light Microscopy: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This product has been referenced in:
Warner A et al. CPNA-1, a copine domain protein, is located at integrin adhesion sites and is required for myofilament stability in Caenorhabditis elegans. Mol Biol Cell24:601-16 (2013).
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