兔Anti-Armenian仓鼠IgG H&L (HRP) (ab5745)

概述

  • 产品名称兔Anti-Armenian仓鼠IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 描述
    兔多克隆抗体二抗to亚美尼亚仓鼠IgG - H&L (HRP)
  • 靶标种属Armenian Hamster
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Armenian Hamster IgG and Armenian Hamster Serum.
  • 经测试应用适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC-Pmore details
  • 免疫原

    Armenian Hamster IgG whole molecule.

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
  • 共轭说明Horseradish Peroxidase (HRP).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/5000.
ELISA 1/25000. This product has been assayed against 1.0 ug of Armenian Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC-P 1/1000 - 1/5000.

Rabbit Anti-Armenian Hamster IgG H&L (HRP) (ab5745)参考文献

This product has been referenced in:
  • Bianchi R  et al. Mutation of Threonine 34 in Mouse Podoplanin-Fc Reduces CLEC-2 Binding and Toxicity in Vivo While Retaining Anti-lymphangiogenic Activity. J Biol Chem 289:21016-21027 (2014). Read more (PubMed: 24907275) »
  • Birjandi SZ  et al. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol 186:3441-51 (2011). Read more (PubMed: 21307289) »

See all 2 Publications for this product

Product Wall

Thanks for your message and for kindly providing this further information.

I have copied below the IHC-P testing protocol for this antibody, which includes the antigen retrieval details. I hope this will be helpful. Please note this protocol...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Vielen Dank für Ihre Anrufe.
Wie besprochen können wir den MCP1 Antikörper für Western blot und Durchflußzytometrie garantieren (www.abcam.com/Abpromise). In der Immuncytochemie/Immunfluoreszenz (ICC/IF) wurde er noch nicht getestet, aber es beste...

Read More

Thank you for your enquiry. This product ab36772 was created by immunizing Armenian hamsters with a synthetic peptide conjugated to KLH. The resulting splenocytes were then fused to the tumor cell line SP2/0. We recommend using any HRP conjugat...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"