兔Anti-Armenian仓鼠IgG H&L (HRP) (ab5745)

概述

  • 产品名称
    兔Anti-Armenian仓鼠IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 描述
    兔多克隆抗体二抗to亚美尼亚仓鼠IgG - H&L (HRP)
  • 靶标种属
    Armenian hamster
  • 特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Armenian Hamster IgG and Armenian Hamster Serum.
  • 经测试应用
    适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC-Pmore details
  • 免疫原

    Armenian Hamster IgG whole molecule.

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
  • 共轭说明
    Horseradish Peroxidase (HRP).
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
WB 1/1000 - 1/5000.
ELISA 1/25000. This product has been assayed against 1.0 ug of Armenian Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent dilution.
IHC-P 1/1000 - 1/5000.

Rabbit Anti-Armenian Hamster IgG H&L (HRP) (ab5745)参考文献

This product has been referenced in:
  • Bianchi R  et al. Mutation of Threonine 34 in Mouse Podoplanin-Fc Reduces CLEC-2 Binding and Toxicity in Vivo While Retaining Anti-lymphangiogenic Activity. J Biol Chem 289:21016-21027 (2014). Read more (PubMed: 24907275) »
  • Birjandi SZ  et al. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol 186:3441-51 (2011). Read more (PubMed: 21307289) »

See all 2 Publications for this product

Product Wall

Thanks for your message and for kindly providing this further information.

I have copied below the IHC-P testing protocol for this antibody, which includes the antigen retrieval details. I hope this will be helpful. Please note this protocol...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Vielen Dank für Ihre Anrufe.
Wie besprochen können wir den MCP1 Antikörper für Western blot und Durchflußzytometrie garantieren (www.abcam.com/Abpromise). In der Immuncytochemie/Immunfluoreszenz (ICC/IF) wurde er noch nicht getestet, aber es beste...

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Thank you for your enquiry. This product ab36772 was created by immunizing Armenian hamsters with a synthetic peptide conjugated to KLH. The resulting splenocytes were then fused to the tumor cell line SP2/0. We recommend using any HRP conjugat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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