概述

  • 产品名称Anti-Rab9抗体
    参阅全部 Rab9 一抗
  • 描述
    兔多克隆抗体to Rab9
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab9.

    (Peptide available as ab45894.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Spleen (Mouse) and Spleen (Rat).

性能

应用

Our Abpromise guarantee covers the use of ab45864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

靶标

Anti-Rab9 antibody 图像

  • All lanes : Anti-Rab9 antibody (ab45864) at 1 µg/ml

    Lane 1 : Mouse spleen tissue lysate - total protein (ab29293)
    Lane 2 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab45864 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45864, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Rab9 antibody (ab45864)参考文献

ab45864 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab45864.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"